Summary
In order to characterize a fibroblast cell line representing normal human skin fibroblasts in three-dimensional cultures, we compared the fibroblast line MSU-1.1, derived from human foreskin and immortalized by v-myc, to primary human dermal fibroblasts (NDF). Our results demonstrate that in contrast to NDF, all MSU-1.1 fibroblasts die within 3–4 d when cultured within three-dimensional contractile collagen matrices. Also, in contrast to NDF, MSU-1.1 cells die markedly in anchored collagen gels as well. Death is due to apoptosis and is attenuated by addition of antibodies against collagen-recognizing receptors α1β1 and α2β1. Apoptosis of NDF in collagen lattices was repressed by an inhibitor of caspase-1, which was ineffective on apoptosis of MSU-1.1. Further, apoptosis by MSU-1.1 fibroblasts was also observed in anchored, i.e., restrained collagen lattices, an environment that supports proliferation of NDF.
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Niland, S., Cremer, A., Herzhoff, K. et al. Apoptosis in v-myc-transfected MSU-1.1 fibroblasts is induced by cell-matrix contact and differs from that of normal dermal fibroblasts. In Vitro Cell.Dev.Biol.-Animal 37, 606–612 (2001). https://doi.org/10.1290/1071-2690(2001)037<0606:AIVMTM>2.0.CO;2
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DOI: https://doi.org/10.1290/1071-2690(2001)037<0606:AIVMTM>2.0.CO;2